P4Gold is a non-invasive 20-minute rapid heat detection test for use in milk. By measuring progesterone levels directly in a cows’ milk, it tells you precisely where she is in her oestrous cycle making it the ideal solution for cows that show no reliable signs or behaviours.
Progesterone testing is 99% accurate in determining whether a cow is in heat/oestrus, making it the
gold standard for Oestrus or Heat detection.1–3
P4Gold is a non-invasive 20-minute rapid heat detection test for use in milk. By measuring progesterone
levels directly in a cows’ milk, it tells you precisely where she is in her oestrous cycle—making it the ideal
solution for cows that show no reliable signs or behaviours.
✓ Confirm true heat
✓ Identify silent heat
✓ Optimise conception timing
✓ Reduce repeat AI services
✓ Shorten calving intervals
✓ Verify wearable data
P4Gold can improve PD percentages, provide insight into poor fertility, help diagnose fertility problems,
and complement other heat detection methods to eliminate false positives. It also functions as a nonpregnancy
test when used after an AI.

Sample: 3 drops of milk
Run: 20 min
Read: Clear Low / Not low
Validated across multiple milk-types, including Jersey. Delivers precise progesterone measurement at
>2 ng/ml, with no cross-reactivity or interference from other reproductive hormones.4
Diagnostic accuracy: sensitivity 100%, Specificity: 98%.4
1. Booth JM, Davies J, Holdsworth RJ. Br. Vet. J.135, 478–488 (1979).
2. Bulman DC, Lamming GE. Br. Vet. J.135, 559–567 (1979).
3. RoelofsJB et al. Anim. Reprod. Sci.91, 337–343 (2006).
4. P4 Science. P4Gold Instructions For Use. 2026.